Nanoparticle-mediated bio-sensing promoted the development of novel sensors in the front of medical diagnosis. A novel biosensor based on microRNA (miRNA) probe functionalized with gold nanoparticles (AuNP) was demonstrated for early detection of Covid-19. Currently, quantitative real-time polymerase chain reaction (qRT-PCR) is being used widely to detect COVID-19, which is time-consuming, labor-intensive and may not be rapidly deployable in remote or resource-limited settings. We aim to improve the sensitivity, selectivity and accuracy of qRT-PCR and Rapid-Test-Kits (RTKs) by applying a direct method using specific extracellular circulating microRNA (miRNA) with electrochemical detection by using nanomaterials-enhanced electrodes. MiRNA hybridization procedure is proposed, where the probe strand would be extended as long as the target strand. We have investigated the variation of ionic conductivity during the fabrication of the ﬁlm and the hybridization using simple pico-ammeter. The present approach provides low detection limit for miRNA in femtomolar, rapid, label-free and easy-to-use virus detection, which holds the potential for future use in various miRNA analyzed by integrated into a self-contained biochip.
Created by: Dr. Nor Azizah binti Parmin, Prof. Ts. Dr. Uda Hashim, Assoc. Prof. Dr. Subash C.B. Gopinath, Muhammad Nur Aiman bin Uda